Table 1 Primary antibodies used by Western Blot analysis
Primary antibody | Host | Dilution | Trading house | Reference |
|---|---|---|---|---|
Akt Total | Rabbit pAb | 1:1000 | Cell Signaling | 9272 |
Akt p-Ser473 | Rabbit pAb | 1:1000 | Cell Signaling | 9271 |
mTOR Total | Rabbit pAb | 1:1000 | Cell Signaling | 2972 |
mTOR p-Ser2448 | Rabbit pAb | 1:750 | Cell Signaling | 2971 |
P70S6K Total | Rabbit pAb | 1:1000 | Cell Signaling | 9202 |
P70S6K p-Thr389 | Rabbit pAb | 1:500 | Cell Signaling | 9205 |
S6 p-Ser240/244 | Rabbit pAb | 1:1000 | Cell Signaling | 2215 |
GFAP | Rabbit pAb | 1:1000 | Cell Signaling | M0761 |
sTREM2 | Rabbit pAb | 1:1000 | Alomone Labs | ANR-018 |
β-Actin | Mouse mAb | 1:2000 | Sigma-Aldrich | A5441 |