Fig. 4

Rapamycin reduces neuronal loss after MCAO. A Representative immunohistochemical images from cortex infarcted area and cortex peri-infarct region (coronal section, 10 µm). Staining was performed used NeuN antibody. Scale bar: 25 µm. B Graphical quantification of NeuN⁺ cell counting across the entire damaged cortical region. C Graphical quantification of NeuN⁺ cell counting, distinguishing between the two damaged regions (white bars represent peri-infarct region whereas red bars represent infarcted area). D Representative Western Blot images of the analysed extracts proteins from mice cerebral cortex. Immunodetections were performed using antibodies against NeuN and B-Actin, as a loading control. E Graphical quantification of immunodetection by Western Blot. Data are normalized against B-Actin and expressed as the percentage of variation versus sham. Graph values represent means ± SEM (Ordinary one/two-way ANOVA * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Unparied t-test ⁺ p ≤ 0.05; ⁺⁺ p ≤ 0.01; ⁺⁺⁺ p ≤ 0.001). Sham + V n = 5, Sham + R n = 5, MCAO + V n = 7; MCAO + Rpre n = 6 and MCAO + R_post n = 5 (V = vehicle; R = rapamycin). * vs Sham + V and ⁺ vs MCAO + V group