Fig. 3

Rapamycin reduce mTORC1 activity in microglia after MCAO. A Representative confocal immunofluorescence images. Staining was performed using Dapi (blue), S6 p-Ser240/244 (green) and Iba-1 (red) antibodies. Scale bar: 50 µm. B Representative Western Blot images of the analysed extracts proteins from mice cerebral cortex. Immunodetections were performed using antibodies against S6 p-Ser240/244 and B-Actin, as a loading control. C Graphical quantification of immunodetection by Western Blot. Data are normalized against B-Actin and expressed as the percentage of variation versus sham. Graph values represent means ± SEM (Ordinary one-way ANOVA * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Unparied t-test ⁺ p ≤ 0.05; ⁺⁺ p ≤ 0.01; ⁺⁺⁺ p ≤ 0.001). Sham + V n = 5, Sham + R n = 5, MCAO + V n = 7; MCAO + Rpre n = 6 and MCAO + R_post n = 5 (V = vehicle; R = rapamycin). * vs Sham + V and ⁺ vs MCAO + V group