Fig. 2

Rapamycin decreases mTORC1 activity in tissue after MCAO A Schematic representation of the mTORC1 pathway. B Representative Western Blot images of the analysed extracts proteins from mice cerebral cortex. Immunodetections were performed using antibodies against total Akt, Akt p-Ser473, total mTOR, mTOR p-Ser2448, total P70S6K, P70S6K p-Thr389 and B-Actin, as a loading control. C–H Graphical quantification of immunodetection by Western Blot. Data are normalized against B-Actin and expressed as the percentage of variation versus sham. Graph values represent means ± SEM (Ordinary one-way ANOVA * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Unparied t-test ⁺ p ≤ 0.05; ⁺⁺ p ≤ 0.01; ⁺⁺⁺ p ≤ 0.001). Sham + V n = 4–5, Sham + R n = 4–5, MCAO + V n = 5; MCAO + Rpre n = 4–5 and MCAO + R_post n = 4–5 (V = vehicle; R = rapamycin). * vs Sham and ⁺ vs MCAO + V group