Fig. 6

Circ_0002669 promotes OS malignancy through a miR-889-3p/MYCBP axis. A, B Cell proliferation and colony formation was determined by CCK-8 and colony formation assays in control, miR-889-3p, miR-889-3p + circ_0002669, si-MYCBP and si-MYCBP + circ_0002669 OS cell subgroups. C Apoptosis was measured by flow cytometry following annexin V-FITC/PI staining in control, miR-889-3p, miR-889-3p + circ_0002669, si-MYCBP and si-MYCBP + circ_0002669 OS cell groups. D, E Cell migration was determined by transwell and wound healing assays in control, miR-889-3p, miR-889-3p + circ_0002669, si-MYCBP and si-MYCBP + circ_0002669 OS cell groups (scale bar, 100 μm). F, G Relative expression of MYCBP, c-Jun, CCND1 and CDK4 mRNA and protein levels was determined by qRT-PCR and western blotting in control, miR-889-3p, miR-889-3p + circ_0002669, si-MYCBP and si-MYCBP + circ_0002669 OS cell groups. H Schematic diagram of this paper. Data shown are from three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001