Fig. 2

Circ_0002669 enhances OS cell proliferation and migration both in vitro and in vivo. A Cell proliferation was quantitated by CCK-8 assay. B Cell colony forming ability was measured. C Apoptosis of OS cells quantitated by flow cytometry via Annexin V-FITC/PI staining. D Expression of apoptosis-related proteins was detected using western blotting in OS cells. E Cell migration of OS cells determined by transwell assays (scale bar, 100 μm). F Migration of OS cells determined by wound healing assay. G Images of xenograft tumors in control and circ_0002669 groups (n = 7). H IHC staining of KI-67 and cleaved caspase-3 in control and circ_0002669 groups (scale bar, 50 μm). I Lung metastasis following tail vein injection of stable circ_0002669-overexpressing or control cells into nude mice. The mice were euthanized at 35 days. HE staining of lungs displayed metastatic nodules (scale bar, 50 μm). Data shown are from three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001