Fig. 6
From: RGS5 maintaining vascular homeostasis is altered by the tumor microenvironment
Tumor microenvironment modulates the pro-inflammatory phenotypic switching of VSMCs by tumor-derived RGS5. (A, B) qRT-PCR and Western Blot analysis the expression of RGS5, TNF-α and VCAM-1 in BC-CM-pre-treated, untreated VSMCs (Con) or RGS5 knockdown BC-CM-pre-treated VSMCs for 24 h followed 24 h of TNF-α stimulation. (C) The fluorescent intensity quantification of BC-CM-pre-treated, untreated VSMCs (Con) or RGS5 knockdown BC-CM-pre-treated VSMCs for 24 h followed calcein-AM-labeled RAW264.7 cell adhesion for 30 min. (D) Representative immunofluorescence images of RGS5 with the pro-inflammatory markers VCAM-1 in TNBCs and Non-TNBC. Scale bar = 100 μm. (E) Lenti-RGS5 was infected into the breast cancer cells. Scale bar = 100 μm. (F) Western blot analysis and quantification of RGS5 expression in the tumor cells after Lenti-RGS5 infection. (G) Representative immunofluorescence images of VSMCs co-incubation with the tumor cells infected with Lenti-RGS5 or not. VSMCs are marked with white arrows. Scale bar = 25 μm. The data were presented as the mean ± SD