Fig. 2

RGS5 ablation contributes to pyroptosis and vascular inflammation. (A) The fluorescent intensity quantification of calcein-AM-labeled RAW264.7 cell adhesion to WT and Rgs5-KO VSMCs treated with TNF-α. (B) Western blot analysis and quantification of RGS5, CD74, GSDMD, GSDMD-N, Caspase-1 and cleaved Caspase-1 in WT and Rgs5-KO VSMCs stimulated with TNF-α for 48 h, respectively. (C) Representative immunofluorescence images of CD86, CD3, MPO, Cleaved-Caspase-1 and N-terminal of GSDMD (NT-GSDMD) stained in ligated carotid arteries for 14 days of WT or Rgs5-KO mice, or Rgs5-KO mice with intravenous injection of AAV-Flag-RGS5 (KO + AAV-RGS5) for 4 weeks. Scale bar = 75 μm. The data were presented as the mean ± SD