Fig. 4
From: Image-based modeling of vascular organization to evaluate anti-angiogenic therapy
Effect of anti-angiogenic treatment on vasculature of tumors. a Segmentation of the perfused vasculature in different tumors including lineup of treatment results with the two anti-angiogenic agents mG6-31 and axitinib. White arrows indicate larger areas void of perfused vessels. Grid scale: 50 µm. b Comparison of RM10 values between tumors and tumors treated with mG6-31 or axitinib. n ≥ 4. c Comparison of relative perfused vessel volume between tumors and tumors treated with mG6-31 or axitinib. n ≥ 4. d Tracing results of perfused vessels in 4T1 tumors after treatment with mG6-31 or axitinib. Vessel segments are displayed color-coded according to average diameters. Treatment did not result in a more homogeneous distribution of vessel diameters, but overall vessel diameter was increased. Grid scale: 50 µm. e Results from PCA of vascular parameters in normal organs and in the three tumor models after anti- angiogenic therapy with either mG6-31 or axitinib. Orientation of four highly ranked selected variables (blue arrows) are shown. Error bars: ± SEM. Asterisks display results from statistical tests with *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001