Fig. 2

Single-cell transcriptomics of organoids. A Single-cell transcriptomics consists of isolation of single cells, followed by lysis, RNA conversion to cDNA by reverse transcription and barcoding. Barcoded cDNA is then used for preparation of cDNA library which can be sequenced and subsequently analysed. B Dimension reduction allows clustering of single cells based on transcriptome profile. Identification of highly expressed marker genes enables cell type annotation. Additional analyses can assess RNA kinetics (pseudotime analysis), cell cycle state, gene expression and pathway enrichment. C Recently, single-cell atlases of human organoid models corresponding different tissues have been established