Fig.2

Effects of the Nef from a primary isolate on erythroid and myeloid cell development in vitro. LVX-vector or nef transduced HSPCs were inoculated into methylcellulose-based media for colony forming assay. After 14 days in culture, numbers of CFU-GM, BFU-E and CFU-GEMM colonies were scored. Data are represented as mean ± SEM in a. These colonies were harvested and some of the harvested cells were stained for CD45/CD235a (erythroid, b and c) and CD45/CD33/CD14 (myeloid, d and e) markers for flow analysis. Representative flow images of erythroid and myeloid lineage cells were shown in b and d, and the cumulative flow data of these two lineage cells were shown in c and e. Data are represented as mean ± SEM. The parental cells in c and e are referred to live cells gated in FSC/SSC plot and CD45 + cells, respectively. The rest of the harvested cells were subjected to cyto-spinning and Giemsa staining, and representative images of these cells are shown in f. ***p < 0.001; **p < 0.01; *p < 0.05