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Fig. 2 | Biology Direct

Fig. 2

From: Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma

Fig. 2

LOXL1-AS1 acts as a ceRNA to sponge miR-3614-5p in HCC cells. A, B Subcellular fractionation assay and FISH assay, supported with bioinformatics analysis (LncLocator), were done to determine the distribution of LOXL1-AS1 in HCC cells. C In RIP assay, the enrichment of LOXL1-AS1 in Ago2 group was measured by qRT-PCR. D qRT-PCR analysis tested the expression of candidate miRNAs in THLE-2, HCCLM3 and SK-HEP-1 cells. E RNA pull down assay was utilized to verify the combination of LOXL1-AS1 and miR-3614-5p. F The binding sites of miR-3614-5p and LOXL1-AS1 were obtained from ENCORI database. G The overexpression efficiency of miR-3614-5p mimics was detected by qRT-PCR. H Luciferase reporter assay validated the binding of miR-3614-5p and LOXL1-AS1 in HCC cells. **P < 0.01

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Biology Direct

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