Fig. 2

Rab32 Knockdown Impairs Starvation-Induced Autophagy. A Representative immunofluorescence images and quantification after a 2 h incubation with EBSS of MCF7 cells stably expressing LC3-GFP with control siRNA or siRNA against Rab32, probed for endogenous Rab32 (red) and nuclei (DAPI, blue) in parallel. Bar indicates 10 µm. Quantification of cells with > 10 LC3 puncta, indicating increased autophagy, were expressed as percent of total cells counted. ≥ 100 cells per condition were counted in each (n = 3) independent experiment. *p ≤ 0.05. B Immunoblot and densitometry analysis of MCF7 cells transfected with control siRNA or siRNA against Rab32, analyzed for LC3II/Tubulin after 4 h EBSS or 48 h 100 nM Bafilomycin A1 incubation. n = 3, **p ≤ 0.001