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Fig. 4 | Biology Direct

Fig. 4

From: Long non-coding RNA LINC00426 contributes to doxorubicin resistance by sponging miR-4319 in osteosarcoma

Fig. 4

MiR-4319 acted as an inhibitor in the OS doxorubicin resistance. a The relative expression level of miR-4319 in doxorubicin-resistant MG63 (MG63/DXR) and KHOS (KHOS/DXR) cells transfected with miR-4319 mimics (miR-4319) or negative control miRNA (miR-NC) was detected by qRT-PCR. b and c Cell counting kit-8 assays were performed to examine the cell proliferation rate of MG63/DXR and KHOS/DXR cells in response to doxorubicin after miR-4319 overexpression. d Clone formation assays were performed to examine cell vitality of MG63/DXR and KHOS/DXR cells to doxorubicin exposure (10 μg/ml, 14 days) after miR-4319 overexpression. e IC50 values of MG63/DXR and KHOS/DXR cells transfected with miR-4319 mimics greatly decreased compared with those of the negative control miRNA (miR-NC) group. f Caspase-3 activity assays were performed to examine cell apoptosis of MG63/DXR and KHOS/DXR cells to doxorubicin exposure (0.1 μg/ml, 48 h) after miR-4319 overexpression. The data represent the mean ± SD. Two-way ANOVA for B and C, Student’s t-tests for others. *P < 0.05; **P < 0.01; ***P < 0.001

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