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Fig. 3 | Biology Direct

Fig. 3

From: Long non-coding RNA LINC00426 contributes to doxorubicin resistance by sponging miR-4319 in osteosarcoma

Fig. 3

LINC00426 acted as a “sponge” of miR-4319 in OS cells. a The prediction for miR-4319 binding sites on LINC00426 transcript and schematic of luciferase reporter vector constructs LINC00426 wild-type (LINC00426-wt) and the miR-4319-binding-site mutated (LINC00426-mut) one. b and c The luciferase activities in MG63/DXR and KHOS/DXR cells co-transfected with miR-4319 or miR-NC mimics and luciferase reporters containing LINC00426-wt or LINC00426-mut. Data are presented as the relative ratio of hRluc luciferase activity to hluc+ luciferase activity. d and e Lysates from MG63/DXR and KHOS/DXR cells were incubated with in vitro-synthesized biotin-labeled sense or antisense DNA probes against LINC00426 for biotin pull-down assay, followed by qRT-PCR analysis to examine LINC00426 and miR-4319 levels. f Detection of LINC00426 in biotinylated miRNA/target complex by real-time RT-PCR. The relative level of LINC00426 in the complex pulled down by using biotinylated miR-4319 (Bio-miR-4319) was compared to that of the complex pulled down by using the biotinylated control random RNA (Bio-miR-NC) in MG63/DXR and KHOS/DXR cells. g The relative expression levels of miR-4319 in MG63/DXR and KHOS/DXR cells transfected with LINC00426 siRNAs (si-LINC00426–1, si-LINC00426–2) or negative control siRNA (si-NC) were detected by qRT-PCR. h The relative expression levels of miR-4319 in MG63/DXR and KHOS/DXR cells transfected with LINC00426 overexpression plasmid (LINC00426-OE) or empty vector (Vector) were detected by qRT-PCR. The data represent the mean ± SD. Student’s t-tests. *P < 0.05; **P < 0.01. ns = not significant

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