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Fig. 5 | Biology Direct

Fig. 5

From: Finite-size effects in transcript sequencing count distribution: its power-law correction necessarily precedes downstream normalization and comparative analysis

Fig. 5

NGS comparative analysis evaluation workflow. The workflow broadly entails the following 4 steps: (i) the read mapping to produce transcript count, (ii) the filtering of the transcripts to ensure non-zero (i.e., no missing) count values between conditions, (iii) the application of a normalization procedure to minimize both bias and variance and finally, (iv) the statistical testing to elucidate significant genes based on some pre-determined p-value and fold-change cutoff. As such, the correction step is best inserted after the filtering step and before the normalization step

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Biology Direct

ISSN: 1745-6150

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