Fig. 4

Lb- and AsCpf1 are equally or more efficient to induce SSA than three tested Cas9 counterparts. Percentages of GFP positive cells counted above the background level, resulted by the action of various nucleases. Six nucleases were tested, each of them on five different targets using the GFxFP assay. Columns show the average values obtained for the five different targets in case of each nuclease. GFP positive cells were counted two days after transfection. All samples were also cotransfected with an mCherry expression vector and the results are analysed within the mCherry positive population. GFP fluorescence for negative controls was measured using an inactive SpCas9 expressing vector (Tálas et al., submitted manuscript) and the obtained values are subtracted from each corresponding sample value. Three parallel transfections were made for each case. Average HDR inducing efficiencies were considered by calculating the average of 15 samples. SpCas9: green, LbCpf1: light green, AsCpf1: yellow, SaCas9: orange, StCas9: red, NmCas9: scarlet