The cyclin D1 carboxyl regulatory domain controls the division and differentiation of hematopoietic cells

Biology Direct

Table 1 Ccnd1 4–5 over-expression increases lymphocyte division

LSKs were purified from 5FU-treated CD45.1 and CD45.2 wild type mice. CD45.1 LSK were transduced with a D1 4–5 coding retrovirus whereas CD45.2 LSK were transduced with the same control (Mock) virus. Equal numbers of each transduced LSK were injected simultaneously into lethally irradiated Rag⁻ mice. 3 weeks after cell transfer, the proliferation rates of the EGFP⁺ CD8⁺T subset were evaluated after 3 i.p. pulses of BrdU. Table 1 shows the % of BrdU⁺ CD8⁺T cells generated from transduced LSKs (EGFP⁺) Mock (CD45.2) and D1 4–5 (CD45.1) in each individual mouse (n = 4). Differences in proliferation between Mock and D1 4–5 transduced lymphocytes were evaluated by the paired Student t-test

ISSN: 1745-6150