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Fig. 9 | Biology Direct

Fig. 9

From: dissectHMMER: a HMMER-based score dissection framework that statistically evaluates fold-critical sequence segments for domain fold similarity

Fig. 9

DissectHMMER analysis of TIP12_MAIZE. The most significant domain hit MIP (PF00230) describes a water/glycerol channel that scores a total FPR of 0.0. Interestingly, the next two domain hits Voltage_CLC (PF00654.15) and Form_Nir_tranp (PF01226.12), marks a jump from the MIP domain hit with a total FPR of 0.29 and 0.35 respectively. Their structurally-derived %Ids are 6.6 and 9.5 respectively. This is concurrently accompanied by a change of the transported solute from water (H2O) to some anions, chloride (Cl−) and nitrite (NO2 −) respectively. The fourth domain ECF-ribofla_trS (PF07155.7) scores a total FPR of 0.61 and structurally-derived %Id of 6.4, and is a 5-TM channel protein that transports riboflavin molecules (generally neutral in charge) across the membrane. Overall, the cohort of domain hits MIP, Voltage_CLC, Form_Nir_tranp and ECF-ribofla_trS generally describes a channel protein that transports its solute across the lipid membrane. In contrast, the last domain hit BCCT (PF02028.12) describes a 12-TM antiporter (concurrent exchange of carnitine and butyrobetaine) that is mechanistically different from a channel protein. This BCCT hit scores an unimpressive total FPR of 1.01. It is further ousted as a positive domain hit by its structural alignment results against TIP12_MAIZE with a bad RMSD of 9.73 and a low %Id of 3.8

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