Figure 5

Sequences of the C-terminus of the Iceberg region of the alto group, with known and predicted membrane-binding regions. Sequence alignment of the last two secondary structure elements of the Iceberg region of the alto group. Conventions are the same as in Figure 3. The penultimate secondary element is αH in all taxa, and the last element is either a β-strand (β1), in alphaviruses, or an α-helix (αI) in other genera (see also Figure 4). The experimentally characterized, amphipathic helix of bromoviruses is doubly underlined. Amphipathic helices predicted by Heliquest are singly underlined. The sequences of each genus have no significant sequence similarity, and were aligned instead according to their secondary structure and hydrophobicity, using Promals [39].