Figure 6

Function similarity analysis. Functional similarity between interacting proteins and the primary and secondary functions of moonlighting proteins. 27 moonlighting proteins in the MPR1-3 sets that have physically interacting proteins in STRING database and their 575 interacting proteins were analyzed. (A) The functional similarity score is computed between GO terms of the primary (F1) or the secondary (F2) functions of a moonlighting protein against the entire GO terms of its interacting protein and the score difference was computed. Interacting proteins were classified by the range of funsim score difference between F1 and F2 GO terms from their interacting moonlighting proteins. (B) The same type of chart as panel A, using the BP-funsim score. (C) For each moonlighting protein, percentages (%) of interacting proteins sharing F1, F2, or both functions of moonlighting proteins are shown. The BP-funsim score was used to determine if proteins share functional similarity. If an interacting protein has a BP-funsim score to both F1 and F2 GO terms of the moonlighting protein, it is classified as both. An interacting protein is considered to share F1, F2, or both functions if the BP-funsim score is larger than the mean SS ^Rel score of BP GO pairs of F1 or F2 in the moonlighting protein. In the case that a moonlighting protein has 0 SS ^Rel score, the cutoff was set to 0.4 for an interacting protein to be considered to share F1, F2, or both functions. P47897 does not have any interacting proteins with F1 or F2 function. Its only interacting protein, RSBN1, has a BP-funsim score of 0 with F1 and F2 functions of P47897. P36024 also does not have any interacting proteins sharing F1 or F2 function. Out of its four interacting proteins, YKL088W has the highest funsim-BP score with F1/F2 GO terms of P36024 (score 0.25), which is below the funsim-BP F1/F2 cutoff for P36024 (cutoff 0.4 for both F1 and F2).