Figure 4

Clustering profiles of sets of moonlighting and non-moonlighting proteins. For each protein in a dataset, GO terms were clustered using various threshold values of SS ^Rel and average number of GO term clusters were plotted. The datasets plotted were experimentally known moonlighting proteins (MPR1, 2, and 3) and identified moonlighting and non-moonlighting proteins in E. coli (Ecoli-MP and Ecoli-nonMP). E. coli moonlighting proteins were also plotted separately for each evidence category, 1 to 3 (Ecoli-PosMP-Cat1-3; see Methods) as well as multi-domain multi-function proteins. (A) BP GO terms were considered. (B) MF GO terms were considered.