A framework for application of metabolic modeling in yeast to predict the effects of nsSNV in human orthologs

Biology Direct

Table 4 Pathway comparison between human candidate proteins and yeast orthologs using KEGG pathway annotations

Human UniProt Ac	Gene name	Pathways	Yeast UniProt Ac	Yeast gene name	Pathway match?	Details
P13716	ALAD	Porphyrin and clorophyll metabolism	P05373	HEM2	Yes	Same
		Biosynthesis of secondary metabolites			No	Pathway only annotated in yeast; same substrate/product
P22830	FECH	Porphyrin and clorophyll metabolism	P16622	HEM15	Yes	Same
		Biosynthesis of secondary metabolites			No	Pathway only annotated in yeast; same substrate/product
P48637	GSS	Glutathione metabolism	Q08220	GSH2	Yes	Same
P49247	RPIA	Pentose phosphate pathway	Q12189	RKI1	Yes	Same
		Biosynthesis of secondary metabolites			No	Pathway only annotated in yeast; same substrate/product
Q96GX9	APIP	Cysteine and methionine metabolism	P47095	MDE1	Yes	Same
Q9Y2Z4	YARS2	Aminoacyl-tRNA biosynthesis	P48527	MSY1	Yes	Same

This table summarizes the pathway involvement of the six identified candidate proteins. For all 6 proteins, the primary pathway involvement is the same, down to the same products and substrates and the same place within a pathway. For three of the yeast proteins, however, the pathway annotation in KEGG listed an additional pathway not annotated for humans: “Biosynthesis of secondary metabolites.” This is likely due to differential annotation between yeast and humans within KEGG. Because the substrates and products annotated for these pathways were identical to those of the primary pathways, these three proteins were not excluded and went onto analysis in the yeast FBA modeling.

ISSN: 1745-6150