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Table 1 Procedures used in Urzymology

From: The Rodin-Ohno hypothesis that two enzyme superfamilies descended from one ancestral gene: an unlikely scenario for the origins of translation that will not be dismissed

Process Remarks
Superimpose homologs POSA server:
ID shared invariant subset Usually 10-30% of monomeric forms
Re-design exposed surface Protein design: Rosetta
Clone & Express Maltose Binding Protein (MBP) fusions; TEV cleavage
Characterize Rate accelerations, substrate specificities
Authenticate Single turnover active-site titration, Steady-state kinetics, genetic mutation and manipulation
Evolutionary recapitulation Multi-scale modular de- and reconstruction