Table 3 Phyletic distribution and associated toxins of Immunity proteins associated with polymorphic toxin systems

Imm-SUKH

α + β (PDB: 3D5P); Several hydrophobic residues and family-specific differences. Refer to previous paper for details

HNH fold families: Tox-SHH,Tox-HNH,Tox-HNH-CIDE, Tox-WHH, Tox-DHNNK, Tox-LHH, Tox-GHH, Tox-HHH, Tox-NucA, Tox-ColE7;

Acidobacteria, actinobacteria, αβγδϵ-proteobacteria, bacteroidetes,, chloroflexi, cyanobacteria, deinococci, firmicutes, fusobacteria, planctomycetes, spirochaetes, synergistetes, verrucomicrobia Eukaryotes: Giardia , ciliophora, choanoflagellida, fungi, Naegleria , metazoa, stramenopiles, viridiplantae, chlorophyta, eukaryotic viruses

This superfamily comprises 5 major families (SUKH1-5), which have been combined in this study; Shows fusions on occasions to toxins and immunity domains; For e.g. fusions to Tox-GHH, Imm-SuFu, Imm33, Imm37, Imm66, Imm67, Imm68, Imm69. Found in homogeneous and heterogenous polyimmunity loci

Restriction endonuclease fold families: Tox-REase-4, Tox-REase-3;

Deaminase families: YwqJ, XOO2897, BURPS668_1122

Proteases: YabG, Tox-PL1; Other toxins: Tox-EndoU, Tox-DOC, Caspase, Tox-ParBL1, Tox-ComI, Ntox15, Ntox20, Tox-ABhydrolase2, Tox-ABhydrolase3

Imm-SuFu

α + β (PDB: 1M1L); GxS, E, E, DxxR

NGO1392-like Tox-HNH fold domain^a (SuFu-associated nuclease), Tox-GHE^b, Tox-ParB^c, Tox-DHNNK ^d, Tox-AHH^e, Tox-HNH^f, Tox-EndoU^g, Tox-EDA39C^h, Tox-PL-C39-like peptidaseⁱ,Tox- ALF-MPTase^j, Ntox7^k

Acidobacteria, actinobacteria^ab,d, α,β^{a,b,c, f},γa^{,c, d},δ^{e, h},ϵ-proteobacteria, bacteroidetes^b,I,k, chloroflexi, firmicutes^b,e,g, fusobacteria, planctomycetes, spirochaetes^j, tenericutes verrucomicrobia.

Fused to members of the SUKH family, ankyrin repeats, Imm5, Imm11, Imm33, Imm36, Imm66, Imm67, Imm68, Imm69, PsbP/MOG1. Found in homo- and heterogeneous polyimmunity loci. See Pfam PF05076

Eukaryotes: chlorophyta, ascomycota, choanoflagellida, metazoa

Imm-SuFu- family 2

α + β; [ST]xxG, [DE]

Tox-ColE7^a, Tox-DHNNK, Tox-HNH fold^b, Tox-ALFMPTase^c,Tox-GDPD^d

actinobacteria α^d,β,γ,δ,ϵ-proteobacteria, bacteroidetes, cyanobacteria, fibrobacteres, firmicutes ^a,b, fusobacteria, gammaproteobacteria, planctomycetes, proteobacteria, spirochaetes^c, verrucomicrobia

Fused to Imm34, Imm33, Imm66, Imm67, Imm68, Imm69; Found in heterogeneous polyimmunity loci

Imm-Cloacin

FKBP-like α + β; EYSxD, NxG

Tox-ColE3^a

Plasmid ^a,ColE6-CT14 ^a, γ-proteobacteria ^a

HEAT repeats

All α;

Tox-REase-7^a

Actinobacteria^a,bacteroidetes,cyanobacteria,γ-proteobacteria,planctomycetes^a,verrucomicrobia^a

Ankyrin repeats (Imm-ank)

All α;

Tox-AHH^a

Firmicutes^a, planctomycetes^a, γ-proteobacteria^a

Fused to SuFu-like immunity domains in firmicutes and found in heterogeneous polyimmunity loci

LRR-repeats

α/β;

Next to T5SS^a toxins

actinobacteria,bacteria,β,γ^a,ϵ-proteobacteria, firmicutes, tenericutes

Found in heterogeneous polyimmunity loci

Imm-CdiI

Two transmembrane helices; several hydrophobic residues

CdiAC

γ-proteobacteria

Imm-NTF2

NTF2 fold (α + β); W, W, W

Tox-NucA ^a

Bacteroidetes, β,γ ^a,ϵ-proteobacteria, firmicutes, fusobacteria, verrucomicrobia

Fused to ankyrin repeats and Imm13 in some proteins

Imm-NTF2-2

NTF2 fold (α + β); Y,W

Tox-JAB-2

γ –proteobacteria (E. coli only)

Although related in structure to Imm-NTF2, the sequences are quite divergent from each other

Imm-PA2201

Two all-α domains(PDB: 2FEF); D, W,GxWxxE, D, YPxD

Tox-REase-1^a, Tox-AHH^b

Bacteroidetes^a, β ^a,γ ^{a, b},ϵ ^a -proteobacteria, firmicutes ^a

See Pfam DUF1910 + DUF1911

Imm-Barstar

α/β (PDB: 1BRS); DxxxD and several hydrophobic residues

Tox-Barnase-like ribonuclease^a

Acidobacteria, actinobacteria^a, α, β^a,γ^a,δ^a,ϵ^a-proteobacteria bacteroidetes^a, chlamydiae^a, chloroflexi^a, cyanobacteria^a, deinococci^a, elusimicrobia, firmicutes^a,fusobacteria^a, nitrospirae^a, planctomycetes^a, verrucomicrobia, Archaea: euryarchaea^a, Eukaryotes: dictyosteliida, Naegleria, chlorophyta

See Pfam PF01337

Imm-ADP-RGHD; ADP ribosyl glycohydrolase

All-α; (PDB: 1t5j); D, D[DE], [RK], H

Tox-ART-RSE^a

acidobacteria, β,γ^a-proteobacteria, firmicutes^a

See Pfam Pf03747; an example of an enzymatic immunity protein

Imm-NMB0513

wHTH fold (α + β, PDB: 2O5H); W, W

Ntox20^a, Ntox7^b

betaproteobacteria^a,b gammaproteobacteria^a

Corresponds to Pfam DUF596

Imm-ComJ

Mostly β; W, F[DE], PF, Y, Y

Tox-ComI-like competence nuclease^a

α^aβ^aγ^a-proteobacteria, bacteroidetes^a, cyanobacteria, firmicutes^a,

Eukaryotes: viridiplantae

Imm-VC0424

α + β; α + β RRM fold, W at C-terminus

-

Firmicutes, fusobacteria, α,β,γ-proteobacteria

Also known as DUF1260 in the Pfam database. Only a subset of members is found in polymorphic toxin systems as potential immunity proteins. These species are listed in column 3

Imm-My6CBD

α + β; E, R, F, W

Tox-ART-HYD1^a

actinobacteria ^a, bacteroidetes ^a, firmicutes ^a, fusobacteria, β ^a,γ ^a –proteobacteria, Eukaryotes : Metazoa

The type VI myosin cargo-binding domain of metazoa appears to have been acquired by lateral transfer from a bacterial version

Imm1

α + β; aromatic and W at C-terminus

SCP1.201 deaminases^a

Actinobacteria^a, bacteroidetes, cyanobacteria, firmicutes, planctomycetes α,β,γ-proteobacteria, verrucomicrobia

Imm2

All α; acidic and hydrophobic residues

BURPS668_1122 deaminases

β, γ- proteobacteria

Imm3

All α; charged, V

BURPS668_1122 deaminases

Firmicutes

found in heterogeneous polyimmunity loci

Imm4

α + β

SCP1.201 deaminases

Burkholderia pseudomallei

Imm5

Mostly α; R, D

DYW deaminases^a, CdiAC^b

Actinobacteria^a, bacteroidetes^a, firmicutes^a, α,β,γ^{a, b},proteobacteria

Fused to Imm36 on occasions

Imm6

Mostly α; P, [DE]

YwqJ deaminases^a

Actinobacteria^a, α-proteobacteria, firmicutes^a

Found in homo and heterogeneous polyimmunity loci

Imm7

α + β; GxaG

Tox-REase-3^a

actinobacteria, firmicutes ^a, planctomycetes

Imm8

α + β; WEa (a:aromatic) at C-terminus

Ntox7^a

Acidobacteria, actinobacteria, bacteroidetes ^a, firmicutes ^a, α, β ^a, γ ^a, δ-proteobacteria

Imm9

α + β; K and several conserved acidic residues

Tox-URI2

Bacteroidetes, γ-proteobacteria

Found in heterogeneous polyimmunity loci

Imm10

Mostly β; R and several hydrophobic residues

Pput_2613 deaminase^a

actinobacteria bacteroidetes chloroflexi firmicutes β, γ^a,δ,ϵ-proteobacteria; Eukaryotes: ascomycetes

Lateral transfer to fungi, found in heterogeneous polyimmunity loci

Imm11

α + β; several conserved hydrophobic residues

Tox-AHH^a, Tox-HNH^b, Tox-SHH^c

Bacteroidetes^a, chloroflexi, cyanobacteria, firmicutes^a, planctomycetes^a, α,β^a,γ^a,δ^a,b,c,ϵ^a-proteobacteria spirochaetes^a verrucomicrobia^a

Listed in the Pfam database as DUF1629. Fused to SuFu on occasions. Found in heterogeneous and homogeneous polyimmunity loci.

Imm12

α + β; several conserved charged and hydrophobic residues

Tox-URI2^a

Bacteroidetes^a, spirochaetes

Found in heterogeneous polyimmunity loci

Imm13

α + β; D, D, D, D

Tox-DOC^a

Actinobacteria, bacteroidetes cyanobacteria, firmicutes, fusobacteria^a, spirochaetes, verrucomicrobia, α,β,γ,δ-proteobacteria,

Note lateral transfer to eukaryotes. Found in heterogeneous polyimmunity loci. Fused to Imm33 in some instances

Eukaryotes: Naegleria

Imm14

Mostly β; several hydrophobic residues

Tox-URI1^a, Tox-HNH^b

Actinobacteria^a, α,β^a,γ^a,,δ^b-proteobacteria, bacteroidetes^a, chlamydiae^a, chloroflexi^a, cyanobacteria, firmicutes^a, fusobacteria, spirochaetes, verrucomicrobia

Found in heterogeneous polyimmunity loci; Fused to Imm51 in one instance

Imm15

α + β; several polar and hydrophobic residues

Bacteroidetes, firmicutes, synergistetes

Found in heterogeneous polyimmunity loci

Imm16

α + β; several hydrophobic residues including a highly conserved W

Ntox8^a

Actinobacteria, bacteroidetes ^a, β ^a,γ,δ-proteobacteria, firmicutes ^a, planctomycetes, spirochaetes, verrucomicrobia

Also known as DUF2750

Imm17

Two TM helices; WxW and a R in the region between them

Bacteroidetes, firmicutes, fusobacteria, spirochaetes

Found in heterogeneous polyimmunity loci

Imm18

Mostly β; highly conserved D

Tox-HNH ^a

Actinobacteria ^a, αβ ^a γ ^a δ ^a -proteobacteria, bacteroidetes ^a, firmicutes

Found in heterogeneous polyimmunity loci

Imm19

α + β; HxxRN motif and several conserved hydrophobic residues

-

Bacteroidetes

Found in heterogeneous polyimmunity loci

Imm20

α + β; several conserved hydrophobic residues

Tox-AHH ^a, Tox-ParB ^b

Acidobacteria, actinobacteria, bacteroidetes, β ^{a, b},γ ^a,δ-proteobacteria, cyanobacterium firmicutes ^a, fusobacteria, planctomycetes, spirochaetes, verrucomicrobia, Eukaryotes: ascomycota

Found in heterogeneous polyimmunity loci. Note presence in ascomycetes

Imm21

α + β; absolutely conserved WxG, YxxxC and several hydrophobic residues

NGO1392-like HNH fold^a

Actinobacteria, α,δ-proteobacteria, bacteroidetes, firmicutes^a, verrucomicrobia

Found in heterogeneous polyimmunity loci

Imm22

α + β; W, Y, and an acidic residue (mostly D)

ColD/E5 fold^a, Tox-REase-4^b, Ntox49^c, Ntox14^d

Actinobacteria, bacteroidetes^a,c, β,γ-proteobacteria, firmicutes ^b,d, fusobacteria, planctomycetes, verrucomicrobia, Eukaryotes: ascomycota

Previously known as SNCF1. Found in heterogeneous polyimmunity loci across a wide range of bacteria

Imm23

α + β; several hydrophobic residues including a WxW motif

Tox-AHH^a, Tox-REase-7^b

bacteroidetes^a cyanobacteria ^b, firmicutes γ-proteobacteria verrucomicrobia

Some versions fused to Imm11; found in heterogeneous polyimmunity loci

Imm24

Mostly α-helical with C-terminal β-hairpin; several hydrophobics including a PxG motif (where x is mostly C)

Tox-AHH^a, Tox-SHH^b

Bacteroidetes^c, β^a,γ^a,ϵ-proteobacteria, firmicutes^a,b, verrucomicrobia

found in heterogeneous polyimmunity loci

Imm25

α + β; highly conserved in limited sequences

-

Bacteroidetes

Potential immunity protein found in heterogeneous polyimmunity loci, and a limited phyletic presence

Imm26

Mostly α; R and D and several hydrophobic residues

Tox-URI1^a

Actinobacteria, bacteroidetes^a, β,γ^a,δ-proteobacteria, firmicutes, fusobacteria, planctomycetes, spirochaetes, Eukaryotes: Ascomycota

Note presence in ascomycetes, present in heterogeneous polyimmunity loci

Imm27

α + β; D, GGxP

Ntox10^a, Tox-ParB^b

Actinobacteria, bacteroidetes ^a, β,δ^b-proteobacteria, verrucomicrobia ^a

Wide distribution but sporadic numbers

Imm28

Mostly α; acidic, P,G, R

Tox-WHH^a, Tox-EndoU^b, Ntox20^c

Actinobacteria, α^a,β^b,c,γ^a-proteobacteria

Note presence in Odyssella, present in heterogeneous polyimmunity loci

Imm29

Mostly α; R and acidic and several hydrophobic residues

Ntox18 ^a

Actinobacteria, α ^a,β ^a,γ ^a -proteobacteria, bacteroidetes, firmicutes, fusobacteria

Note presence in Odyssella, present in heterogeneous polyimmunity loci

Imm30

Mostly α; Several conserved hydrophobics and DxG motif

Tox-SHH^a

α ^a,β^,γ ^a –proteobacteria

Note presence in Odyssella. Limited number of hits, present in heterogeneous polyimmunity loci

Imm31

All-β; GxS, [R]

Ntox17^a, Ntox7^b

α ^a,β^b,γ ^a,δ-proteobacteria, cyanobacteria

Note presence in Odyssella. Limited distribution

Imm32

α + β; H, and several conserved residues

Ntox12^a, Ntox37 ^b, Ntox7 ^c

α ^a,β,γ ^a,c,δ-proteobacteria, chlamydiae, bacteroidetes ^b, firmicutes ^a, verrucomicrobia

Note presence in Odyssella, chlamydiae. Limited distribution

Imm33

Mostly β; W

Tox-HNH ^a, Tox-DHNNK ^b,^, NGO1392-like- HNH^c

Acidobacteria, actinobacteria, αβ ^a,c γδ^c-proteobacteria, bacteroidetes, chloroflexi, firmicutes, ^b, fusobacteria, planctomycetes, Eukaryotes: dictyosteliida

Also known as DUF2185 in the Pfam database, fused to Imm- SUKH, Imm13, Imm34 and Imm-SuFu, Note presence in dictyosteliida where it is fused to Imm34, present in homo and heterogeneous polyimmunity loci

Imm34

Mostly β; ExxW, C-terminal D

-

Actinobacteria, α,β,γ,δ,ϵ-proteobacteria, bacteroidetes, firmicutes, fusobacteria, planctomycetes, spirochaetes, verrucomicrobia, Eukaryotes: dictyosteliida, heterolobosea, cnidaria

Also known as DUF2314. Fused to Imm-SuFu family 2, Imm33, ankyrin repeats, TM helices, fusion to Imm33 appears to have occurred on multiple occasions independently, present in heterogeneous polyimmunity loci. Note presence in Naegleria, dictyosteliida and cnidarians. In dictyostellids, it is fused to Imm33

Imm35

α + β; W, [ST]

Tox-PL1^a, Ntox40^b

Actinobacteria^{a, b}, bacteroidetes^a, β,γ^a-proteobacteria, planctomycetes

Fused to Papain-like toxin and ADP-ribosyl glycohydrolase and Peptidase S8, in some instances. Possible protease inhibitor

Imm36

BH3703-like fold (α + β); W, W

Tox-NucA^a, DYW-Deaminase^b, Ntox40^c, Tox-CdiAC^d, Tox-Caspase^e

Actinobacteria^{a, c, e}, α^a,β^a,γ^a,d,δ-proteobacteria, bacteroidetes^a,b, firmicutes^a, fusobacteria, spirochaetes^a

Also known as DUF600, fused to Tox-NucA, Imm-SuFu, Imm5, on occasions. Tox-NucA appears to be the primary toxin association. One of the large families. Found in homo and heterogeneous poly-immunity loci. Profile-profile analysis predicts a BH3703-like fold

Imm37

α + β; ExG

Tox-WHH^a

Acidobacteria, actinobacteria, αβγ^aϵ-proteobacteria, bacteroidetes, chloroflexi, cyanobacteria, deinococci, firmicutes^a, fusobacteria^a, planctomycetes, verrucomicrobia

Previously known as SNCF2, fused to SUKH in some instances. Found in heterogeneous polyimmunity loci

Imm38

Mostly α; W at N and aromatic residue at C

Ntox19^a, NGO1392-like- HNH^b

Actinobacteria, bacteroidetes ^a, β ^a,b,γ ^a,δ ^a -proteobacteria, firmicutes ^a, fusobacteria ^a, nitrospirae

Also known as DUF2247. Found in heterogeneous polyimmunity loci

Imm39

α + β; GR, GxK and several polar and hydrophobic residues

Tox-URI2 ^a

α ^a γ ^a-proteobacteria

Limited distribution

Imm40

α + β; GGD, F, W

Ntox19^a

bacteroidetes^a, chloroflexi firmicutes, β^a,ϵ,γ^a- proteobacteria

Imm41

α + β; SF, W and several hydrophobic residues

Ntox21^a, Ntox29 ^b, Tox-ART-RSE ^c

Actinobacteria, β ^a,b,γ^c,ϵ-proteobacteria, firmicutes, planctomycetes

Found in homo- and heterogeneous polyimmunity loci

Imm42

α + β; Several conserved hydrophobic residues

Ntox18^a

α,β ^a,γ ^a -proteobacteria, firmicutes ^a

Imm43

α/β; W, P, D, S, R

Tox-AHH^a

Bacteroidetes^a, β-proteobacteria^a, firmicutes

Found in heterogeneous polyimmunity loci

Imm44

α + β; Multiple polar and hydrophobic residiues

Tox-URI1^a, Tox-URI2^b, Tox-ParBL1^c

Bacteroidetes, β-proteobacteria^a,b, firmicutes^c

Limited phyletic distribution; Found in heterogeneous polyimmunity loci that show variations in structure even between closely related strains

Imm45

α + β; C-terminal W

Tox-ColE3^a

bacteroidetes,β ^a,γ ^a,ϵ-proteobacteria, firmicutes

Imm46

α + β; E, W, E

-

Bacteroidetes, β-proteobacteria

Limited phyletic distribution. Found next to a predicted toxin

Imm47

α + β; KxGDxxK

-

β-proteobacteria, firmicutes

Found in heterogeneous polyimmunity loci

Imm48

All-α; HRG

-

Firmicutes,verrucomicrobia

Found in heterogeneous polyimmunity loci

Imm49

All α; Hydrophobic residues, P

Tox-REase-1^a, Tox-REase-6^b

Actinobacteria ^b, Bacteroidetes ^a,b, cyanobacteria ^b, firmicutes ^a, fusobacteria ^a, planctomycetes, β ^a,b,δ,γ ^a,b -proteobacteria

Also known as DUF556

Imm50

Mostly β; Several hydrophobic residues

Tox-HHH^a, Ntox24^b

actinobacteria, bacteroidetes^a, firmicutes^a, planctomycetes, α,β^a,b,γ^a-proteobacteria, verrucomicrobia

Imm51

α + β; W, Dx[DE] and several hydrophobic residues

Tox-RES^a, Tox-URI1^b

Actinobacteria, bacteroidetes^a, β,γ-proteobacteria, cyanobacteria,firmicutes ^b, fusobacteria, spirochaetes

Fused to Imm14 on one occasion, Found in polyimmunity loci

Imm52

α + β; W,GT,F

Tox-REase-5^a

Caudoviruses^a, α,β ^a,γ ^a,δ ^a –proteobacteria

Imm53

α + β (Central β-sheet with flanking α-helices); W, WE, PGW, W

Ntox24^a, Ntox10^b

Acidobacteria, actinobacteria, α,β,γ,δ,ϵ-proteobacteria, bacteroidetes, chlamydiae ^b, cyanobacteria, firmicutes ^a, spirochaetes, verrucomicrobia

Imm54

α + β; GF, Q

Tox-REase-9^a, Tox-RelE^b, Tox-URI^c, Tox-REase-4^d, Tox-REase-7^e, Tox-REase-10^f

actinobacteria, bacteroidetes ^{a, c, d}, chlamydiae ^a, firmicutes ^{a, c, d,e}, fusobacteria^b,f, planctomycetes, α,β ^c,γ ^a,δ,ϵ-proteobacteria, spirochaetes, verrucomicrobia

Found in heterogeneous polyimmunity loci

Imm55

α + β; G and several hydrophobic residues

Tox-SHH^a

actinobacteria, bacteroidetes^a, cyanobacteria^a, firmicutes^a, lentisphaerae, planctomycetes, α,β,γ^a-proteobacteria, synergistetes, verrucomicrobia

Imm56

α + β; D, GR

Ntox38^a, Tox-HNH^b

Actinobacteria ^a,b,

chloroflexi ^a

Imm57

Mostly α; D, SE, C

Tox-LD-peptidase^a, Tox-Caspase^b

β^a,γ^{a, b}-proteobacteria

Imm58

α + β; YxxxD, WxG, KxxxE

Unknown toxins with RHS repeats

β,δ -proteobacteria

Limited distribution

Imm59

α + β (Central β-sheet with flanking α-helices); [DE]R motif

Ntox13^a, Ntox40^b

firmicutes ^a,b

Fused to Imm63 on some instances

Imm60

Mostly β; N, W

Ntox40 ^a, Ntox48^b

bacteroidetes

Found in heterogeneous polyimmunity loci

firmicutes ^a, fusobacteria, α ^b,γ^b –proteobacteria,

euryarchaea

Imm61

α + β; R

Ntox40^a

actinobacteria ^a

Imm62

α + β; -(mostly E), W

Ntox31^a, Ntox48^b

Firmicutes^{a, b},

Found in heterogeneous polyimmunity loci

γ-proteobacteria

Imm63

α + β; E + G, -(mostly E)xxY

Ntox40^a, Tox-CdiAC^b, Tox-ARC

actinobacteria ^a,c

Found in polyimmunity loci

bacteroidetes

firmicutes^a, β,γ^a,b -proteobaceria

Imm64

α + β; DxEA, R motifs

Tox-ColD^a

Euryarchaea^a, firmicutes^a, ϵ-proteobacteria

Imm65

α + β; YxC, and several charged residues

Tox-JAB-1

Bacteroidetes

Contains a signal peptide and a lipbox

Imm66

Mostly α; D, W, F, Y,W

Tox-ABhydrolase3^a, Ntox48^b

Actinobacteria, bacteroidetes, cyanobacteria, firmicutes

Fused to one or more immunity domains such as Imm68, SUKH, Imm-SuFu- family 2, Imm33, Imm69, Imm67, Imm-SuFu, Imm66, and TPR repeats. Some proteins in firmicutes have up to 10 immunity domains

Fusobacteria, α,β ^a,γ^b,ϵ-proteobacteria, spirochaetes, verrucomicrobia, Eukaryotes: Ascomycota, viridiplantae

Imm67

α + β; W, E, W

-

actinobacteria, bacteroidetes, chloroflexi, cyanobacteria, firmicutes, fusobacteria, planctomycetes, α,β,γ,δ, ϵ-proteobacteria, spirochaetes, verrucomicrobia

Fused to one or more immunity domains such as Imm68, Imm33, Imm-SUKH, Imm-SuFu-family 2, Imm69, Imm-SuFu, Imm66, Imm67, TPR and ankyrin repeats. Some proteins in firmicutes have up to 10 immunity domains

Imm68

α + β; E

-

actinobacteria, bacteroidetes, firmicutes, spirochaetes

Fused to one or more immunity domains such as Imm-SUKH, Imm-SuFu, Imm67, Imm66, Imm-SuFu-family 2, Imm69, Imm33, Imm68 andTPR repeats. Some proteins in firmicutes have up to 10 immunity domains

Imm69

α + β; W,hGE(h: hydrophobic)

Tox-ABhydrolase3^a

Actinobacteria, bacteroidetes, firmicutes ^a, fusobacteria, planctomycetes, α,β.γ,ϵ-proteobacteria,, spirochaetes, verrucomicrobia

Fused to one or more immunity domains such as Imm68, Imm-SUKH, Imm33, Imm-SuFu-family 2, Imm-SuFu, Imm67, Imm66, SP, Imm69 and TPR repeats. Some proteins in firmicutes have up to 10 immunity domains

Imm70

α + β; Y,W

Tox-REase-10^a

Acidobacteria, actinobacteria, bacteroidetes, firmicutes^a, β^a,γ^a,ϵ^a-proteobacteria, spirochaetes^a, verrucomicrobia

Imm71

Mostly α; R,F, R

Ntox48^a

acidobacteria ^a, β ^a,γ ^a -proteobacteria

Often fused to Imm72

Eukaryotes: viridiplantae

Imm72

All-β; GxxE, WxDxRY, E

Ntox48^a

acidobacteria ^a, β ^a,γ ^a -proteobacteria

Often fused to Imm71

Imm73

All-α; Several hydrophobic residues

Tox-PL-2^a, Tox-HNH^b

acidobacteria, actinobacteria^b, bacteroidetes, cyanobacteria ^a, firmicutes ^a, fusobacteria, β,γ,δ ^a -proteobacteria, verrucomicrobia

Sometimes found in 2–3 tandem copies in a polypeptide

Imm74

α + β; G[DE], [DE]

Tox-Arc^a

bacteroidetes^a, firmicutes^a, planctomycetes, α,β,γ^a,δ -proteobacteria,

Found in heterogeneous polyimmunity loci