Figure 2

Sequences of Gau proteins from representative taxa of alpha-proteobacteria and eukaryotes. For each nuclear or mtDNA sequence, the specific genetic code has been used. Due to the AGG and AGA stop codons in the mitochondrial vertebrate genetic code (Homo sapiens), the Gau protein sequence has been deduced from a cox1 nuclear pseudogene. Taxa with accession numbers, alpha-proteobacteria: Rhodobacter sphaeroides (CP000661.1) (the gau region is complementary to the sequence from nucleotide position 557002 to 556700), Rickettsia conorii (NC_003103), Wolbachia endosymbiont of Drosophila melanogaster (AE017196); protists: Monosiga brevicollis (NC_004309), Rhodomonas salina (NC_002572); Prototheca wickerhamii (NC_001613); Viridiplantae: Triticum aestivum (NC_007579), Pseudendoclonium akinetum (NC_005926); fungi: Saccharomyces cereviseae (NC_001224), Schizophyllum commune (NC_003049); metazoa: Metridium senile (NC_000933), Drosophila melanogaster (NC_001709), Homo sapiens (NC_001807) (human mt-Gau deduced protein matches from base positions 6288 to 6590) and a human nuclear pseudogene (Homo sapiens (ψ)) (ensemble.org, chromosome: NC_000001.9, gau region matches from the complementary to the sequence from nucleotide positions 557002 to 556700). X, UAA or UAG stop codons; X, UGA stop codon; #, AGA or AGG stop codons; *, identical amino acids;:, similar amino acids. All sequence alignments were performed using ClustalW (http://www.ebi.ac.uk/Tools/clustalw2/index.html). The monoclonal antibody was raised against a synthetic peptide that was derived from the underlined region in the human nuclear pseudogene sequence. Positive and hydrophobic amino acids are in bold letters in the positively charged and hydrophobic domains, respectively.