Figure 5

Three-dimensional conservation grading of ISG20 (A) and RAB30 (B). Two 3-D backbone structures of ISG20 and RAB30 were retrieved from PDB code 1WLJ and 2EW1, respectively. (A) The putative conservation grading was based on the alignment of twenty mammalian protein sequences from: Human (Homo sapiens), Chimpanzee (Pan troglodytes), Orangutan (Pongo pygmaeus), Gorilla (Gorilla gorilla), Macaque (Macaca mulatta), Cow (Bos taurus), Dog (Canis familiaris), Horse (Equus caballus), Cat (Felis catus), Guinea Pig (Cavia porcellus), Mouse (Mus musculus), Rat (Rattus norvegicus), Megabat (Pteropus vampyrus), Microbat (Myotis lucifugus), Pika (Ochotona princeps), Hyrax (Procavia capensis), Tree Shrew (Tupaia belangeri), Dolphin (Tursiops truncatus), Opossum (Monodelphis domestica), Platypus (Ornithorhynchus anatinus). (B) These conservation grades were based on the aligned twenty-two mammalian protein sequences from Human (Homo sapiens), Cow (Bos taurus), Dog (Canis familiaris), Guinea Pig (Cavia porcellus), Horse (Equus caballus), Cat (Felis catus), Elephant (Loxodonta africana), Macaque (Macaca mulatta), Mouse Lemur (Microcebus murinus), Opossum (Monodelphis domestica), Mouse (Mus musculus), Microbat (Myotis lucifugus), Pika (Ochotona princeps), Platypus (Ornithorhynchus anatinus), Rabbit (Oryctolagus cuniculus), Chimpanzee (Pan troglodytes), Orangutan (Pongo pygmaeus), Hyrax (Procavia capensis), Megabat (Pteropus vampyrus), Rat (Rattus norvegicus), Tree shrew (Tupaia belangeri), Dolphin (Tursiops truncatus). The color bars from the left to the right measure changes from variable to conserved residues. Conservation grading in yellow indicates the residues whose conservation degrees were not supported with sufficient data.