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Table 1 Differentially regulated proteins (on pH scales pH 3-10 and pH 4-7) identified by MALDI-TOF MS.

From: Proteomic changes associated with deletion of the Magnaporthe oryzae conidial morphology-regulating gene COM1

SpotA Accession no.B Protein name Mr (KDa)/pIC Hit ratioD Mascot score Sequence Coverage %E
1 gi|39940362 Scytalone dehydratase 20.2/6.25 6/9 80 52
2 gi|39971339 Actin related protein -2 44.7/6.2 16/19 166 51
3 gi|58257439 Conserved hypothetical protein MGG_02610.5 37.7/4.76 9/10 125 50
4 gi|3287916 Tetrahydroxynaphthalene reductase 30/6.67 18/20 202 72
5 gi|39973539 Glyceraldehyde-3-phosphate dehydrogenase 35.9/6.91 13/14 171 64
6 gi|39960255 Malate dehydrogenase 35.1/8.31 16/16 214 62
7 gi|145608532 Glutaryl-CoA dehydrogenase 46/8.37 13/15 156 53
8 gi|39976425 Predicted protein MGG_05861.5 42.8/7.79 19/22 182 59
9 gi|39974499 Beta-tubulin 49.9/4.51 11/12 126 36
10 gi|145608354 ATP synthase beta chain, mitochondrial 55.8/5.27 15/17 162 47
11 gi|145609776 Hypothetical protein MGG_02992.5 53.3/6.31 14/18 124 41
12 gi|145602787 Hypothetical protein MGG_09874.5 22.9/4.98 8/9 120 68
13 gi|39944616 Predicted protein MGG_04319.5 16.8/4.79 4/5 69 52
14 gi|39968283 Hypothetical protein MGG_02234.5 14.8/4.83 3/3 46 19
15 gi|39946616 SMN family protein Smn1 [Schizosaccharomyces pombe] 18.3/4.74 6/6 103 60
16 gi|39975841 Molybdopterin binding domain protein 32.8/5.50 9/10 109 44
17 gi|7051772 Ran GTPase binding protein Mog1 [Schizosaccharomyces japonicus] 21.8/4.48 7/10 92 55
18 gi|39943944 Hsp70 nucleotide exchange factor fes1 23.4/4.26 4/5 52 22
19 gi|39976891 Adenylylsulfate kinase 23.2/6.69 6/8 75 45
20 gi|39970057 Predicted protein MGG_10637.5 36.1/4.87 12/14 144 54
21 gi|145601361 ATP25 protein [Saccharomyces cerevisiae] 31.2/4.95 7/9 92 45
22 gi|145604182 Hypothetical protein MGG_10674.5 44.9/6.66 13/16 141 44
23 gi|39971327 Hypothetical protein MGG_10684.5 37.2/4.85 9/11 106 37
24 gi|145616429 Hypothetical protein MGG_07401.5 39.3/5.08 11/12 140 49
25 gi|145603364 Serine/threonine protein phosphatase PP2A catalytic subunit 36.8/4.59 14/14 195 67
26 gi|39945404 Cytochrome P450 phenylacetate 2-hydroxylase 58.3/6.52 19/20 194 50
27 gi|39970147 Isovaleryl-CoA dehydrogenase-like protein 50.4/7.0 13/18 125 44
28 gi|8102490 Cell cycle control protein (Cwf8), putative [Talaromyces stipitatus]. 51.7/4.95 12/14 126 36
29 gi|145614390 Vacuolar ATP synthase subunit H 53.6/5.04 18/22 179 55
30 gi|145613273 DNA replication licensing factor mcm3 [Verticillium albo-atrum] 58.4/4.84 17/22 151 45
31 gi|240110283 DHHC zinc finger domain containing protein [Coccidioides posadasii] 56.4/5.41 12/13 143 42
  1. PMF data were utilized for protein identification by analyzing the size of tryptic fragments via the MASCOT (Matrix Science) search engine using the entire NCBI fungal protein database.
  2. A) Spot: the numbers of proteins on gels.
  3. B) Accession no.: the number of the predicted protein in NCBI-NR protein database.
  4. C) Calculated from the M. grisea 70-15 sequence using the ExPASy Compute pI/Mw tool.
  5. D) Hit ratio: Number of peptide masses matched/number of masses submitted to the peptide mass fingerprint database search.
  6. E) Per cent of protein sequence covered by matched peptides (PMF).