Proteomic changes associated with deletion of the Magnaporthe oryzae conidial morphology-regulating gene COM1

Bhadauria, Vijai; Wang, Li-Xia; Peng, You-Liang

doi:10.1186/1745-6150-5-61

Biology Direct

Table 1 Differentially regulated proteins (on pH scales pH 3-10 and pH 4-7) identified by MALDI-TOF MS.

From: Proteomic changes associated with deletion of the Magnaporthe oryzae conidial morphology-regulating gene COM1

Spot^A	Accession no.^B	Protein name	Mr (KDa)/pI^C	Hit ratio^D	Mascot score	Sequence Coverage %^E
1	gi\|39940362	Scytalone dehydratase	20.2/6.25	6/9	80	52
2	gi\|39971339	Actin related protein -2	44.7/6.2	16/19	166	51
3	gi\|58257439	Conserved hypothetical protein MGG_02610.5	37.7/4.76	9/10	125	50
4	gi\|3287916	Tetrahydroxynaphthalene reductase	30/6.67	18/20	202	72
5	gi\|39973539	Glyceraldehyde-3-phosphate dehydrogenase	35.9/6.91	13/14	171	64
6	gi\|39960255	Malate dehydrogenase	35.1/8.31	16/16	214	62
7	gi\|145608532	Glutaryl-CoA dehydrogenase	46/8.37	13/15	156	53
8	gi\|39976425	Predicted protein MGG_05861.5	42.8/7.79	19/22	182	59
9	gi\|39974499	Beta-tubulin	49.9/4.51	11/12	126	36
10	gi\|145608354	ATP synthase beta chain, mitochondrial	55.8/5.27	15/17	162	47
11	gi\|145609776	Hypothetical protein MGG_02992.5	53.3/6.31	14/18	124	41
12	gi\|145602787	Hypothetical protein MGG_09874.5	22.9/4.98	8/9	120	68
13	gi\|39944616	Predicted protein MGG_04319.5	16.8/4.79	4/5	69	52
14	gi\|39968283	Hypothetical protein MGG_02234.5	14.8/4.83	3/3	46	19
15	gi\|39946616	SMN family protein Smn1 [Schizosaccharomyces pombe]	18.3/4.74	6/6	103	60
16	gi\|39975841	Molybdopterin binding domain protein	32.8/5.50	9/10	109	44
17	gi\|7051772	Ran GTPase binding protein Mog1 [Schizosaccharomyces japonicus]	21.8/4.48	7/10	92	55
18	gi\|39943944	Hsp70 nucleotide exchange factor fes1	23.4/4.26	4/5	52	22
19	gi\|39976891	Adenylylsulfate kinase	23.2/6.69	6/8	75	45
20	gi\|39970057	Predicted protein MGG_10637.5	36.1/4.87	12/14	144	54
21	gi\|145601361	ATP25 protein [Saccharomyces cerevisiae]	31.2/4.95	7/9	92	45
22	gi\|145604182	Hypothetical protein MGG_10674.5	44.9/6.66	13/16	141	44
23	gi\|39971327	Hypothetical protein MGG_10684.5	37.2/4.85	9/11	106	37
24	gi\|145616429	Hypothetical protein MGG_07401.5	39.3/5.08	11/12	140	49
25	gi\|145603364	Serine/threonine protein phosphatase PP2A catalytic subunit	36.8/4.59	14/14	195	67
26	gi\|39945404	Cytochrome P450 phenylacetate 2-hydroxylase	58.3/6.52	19/20	194	50
27	gi\|39970147	Isovaleryl-CoA dehydrogenase-like protein	50.4/7.0	13/18	125	44
28	gi\|8102490	Cell cycle control protein (Cwf8), putative [Talaromyces stipitatus].	51.7/4.95	12/14	126	36
29	gi\|145614390	Vacuolar ATP synthase subunit H	53.6/5.04	18/22	179	55
30	gi\|145613273	DNA replication licensing factor mcm3 [Verticillium albo-atrum]	58.4/4.84	17/22	151	45
31	gi\|240110283	DHHC zinc finger domain containing protein [Coccidioides posadasii]	56.4/5.41	12/13	143	42

PMF data were utilized for protein identification by analyzing the size of tryptic fragments via the MASCOT (Matrix Science) search engine using the entire NCBI fungal protein database.
A) Spot: the numbers of proteins on gels.
B) Accession no.: the number of the predicted protein in NCBI-NR protein database.
C) Calculated from the M. grisea 70-15 sequence using the ExPASy Compute pI/Mw tool.
D) Hit ratio: Number of peptide masses matched/number of masses submitted to the peptide mass fingerprint database search.
E) Per cent of protein sequence covered by matched peptides (PMF).

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ISSN: 1745-6150

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