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Figure 4 | Biology Direct

Figure 4

From: Issues associated with assessing nuclear localization of N-terminally unphosphorylated β-catenin with monoclonal antibody 8E7

Figure 4

Detection of ABC depends on antigen retrieval method. A-D Rat lung paraffin sections. Sections treated with boiling Tris-Urea (A-C) or sodium citrate buffer (D) before incubation with the following antibodies: (A) GAM-HRP secondary alone, (B) anti-TL-β-catenin, (C&D) anti-ABC (8E7). Note that sections treated with Tris-Urea buffer unmask an epitope detected by ABC and present in all nuclei (C). Sections treated with sodium citrate buffer show diminished nuclear background, and instead reveal a sub-population of lung airway epithelial cells that contain the ABC epitope (arrows; D).

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