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Figure 5 | Biology Direct

Figure 5

From: The control of CD4+CD25+Foxp3+regulatory T cell survival

Figure 5

Foxp3 repression of IL-2 determines the IL-2 dependence in T reg cells. (a) Expression of CD25 on splenocytes isolated from WT or Scurfy mice ex vivo (gated on CD4+ T cells). (b) Expression of intracellular Foxp3 in splenocytes from WT or Scurfy mice showing the frequency of Treg cells. (c) Viability of Tcon or CD25+ cells from WT or Scurfy mice stimulated for 3 days with soluble anti-CD3 and anti-CD28 with or without IL-2 added at 20 ng/ml concentration. (d) Viability of Tresp cells from WT mice stimulated for 3 days with soluble anti-CD3 and anti-CD28 and co-cultured with Tcon cells or CD25+ cells from WT or Scurfy mice. (e) ELISA quantification of IL-2 in Tcon (open bars) or CD25+ (solid bars) cells from WT or Scurfy mice, stimulated for 3 days with soluble anti-CD3 and anti-CD28.

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