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Table 4 Diversity estimate summation of side-by-side comparisons of enzyme/buffer system, cycling parameters and PCR methodology in two 'Amakihi infected with isolate K1 and KV115.

From: Genetic characterization of Hawaiian isolates of Plasmodium relictumreveals mixed-genotype infections

Bird ID Isolate

'Amakihi 6 K1 Kauai

'Amakihi 11 KV115 Hawaii

Enzyme

Promega

  

Roche

Promega

  

Roche

Methods

Standard

PCR+1

  

Standard

PCR+1

  

# cycles

80

80

35

35

80

80

35

35

 

Trial 1

Trial 2

Trial 3

Trial 4

Trial 1

Trial 2

Trial 3

Trial 4

Haplotype 4

  

1

     

12

 

1

      

25

 

1

    

1

 

27

 

2

 

1

    

33

1

       

45

     

1

  

47

      

1

 

49

    

1

1

  

56

      

1

 

58

 

1

      

59

       

2

62

  

1

  

3

  

77

4

11

13

19

5

8

15

16

Haplotypes

2

5

3

2

2

4

4

2

Total # Clones

5

16

15

20

6

13

18

18

Freq. Hap 77

0.8

0.69

0.87

0.95

0.83

0.62

0.83

0.89

D (Simpson's)

1.47

2

1.32

1.1

1.38

2.25

1.42

1.24

H (S-W)

0.5

1.04

0.49

0.2

0.45

1.03

0.63

0.35

Hap/Clone Ratio

0.40

0.31

0.20

0.10

0.33

0.31

0.22

0.11

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