Figure 2

Equivalent expansion and activation of islet specific CD8 T cells in the panLN of Idd9 congenic and NOD mice. CFSE-labeled splenocytes (20 million cells) from 4–6 week old 8.3NODScid donor mice were transferred into age-matched 6–9 week old Idd9 congenic and NOD recipients. On day 4 following transfer panLN and ingLN cells were stained for analysis by flow cytometry. Donor islet-specific CD8 T cells were defined by gating on CFSE+CD8+ cells (A). The percentage of CFSE+CD8+ T cells recovered from the panLN of Idd9 congenic and NOD mice is shown in (B). Representative CFSE profiles of CFSE+CD8+ cells in the panLN and ingLN, with the average % divided cells +/- SEM, are shown in (C). We also stained for the activation markers CD44 (D) and CD11a (E), and representative histograms (gated on CFSE+CD8+ cells) are shown for Idd9 congenic and NOD panLN and ingLN cells, indicating the average value +/- SEM. Data is representative of 4 independent experiments n = 5–6 mice for each experiment.