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Figure 1 | Biology Direct

Figure 1

From: The ability of natural tolerance to be applied to allogeneic tissue: determinants and limits

Figure 1

Pre-immunocompetence single minor-H mismatched transplants are not ignored but instead trigger immunity or natural tolerance depending on the graft site. (A) Experimental design and timeline; (B) Male specific killing for spleen cells from individual mice is depicted. B10-RAG female recipients were given a B10-RAG male or female cardiac graft under the kidney capsule or under the skin of the ear pinna. Less than 10 days (circles) or 6 mos. (squares) later these mice were reconstituted with fetal liver cells from female B6 fetuses. 4–5 mos. post reconstitution they were hemisplenectomized and tested for CTL priming to the H-Y antigen (1st CTL; solid symbols; Male heart vs. female heart: in the ear, p < 0.001, and in the kidney, p > 0.05). Some of the mice were later immunized i.p. with 3 × 106 B6 male spleen cells and 6 or more wks after immunization (9–10.5 mos. after FL) they were tested again for CTL priming to the H-Y antigen (2nd CTL; open symbols; for grafts in the kidney: pre- vs. post-immunization, female heart, p < 0.001, male heart, p > 0.05; post-immunization male vs. female heart, p < 0.01).

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