Codon insertion and deletion functions as a somatic diversification mechanism in human antibody repertoires

Biology Direct

Table 2 Antigen specificity, V-gene usage, and location of codon insertions and/or deletions in individual antigen-specific Fabs

					Number of Codons
Specificity^a	Donors^b	Clone	Isotype	V gene^c	Ins/Del	Location^d
PA	1/1	001.46F1	κ/G1	A27	1 del	CDR1
PPS 6B	3/6	003.6F2	κ/G2	VH3-15	2 ins	CDR2
		023.16B11	λ /A2	VH3-23	4 del	CDR3
		010.3H11	κ/A2	B3	6 del	CDR1
		023.20H2	λ/A2	V1-4	1 ins	FR2
PPS 14	2/3	023.14H10	λ/G2	VH1-46	1 del	CDR2
		023.9E5	λ/G2	VH1-46	2 del	CDR2
		011.11A11	λ/G2	VH3-7	1 ins	FR1
		011.5G1	κ/A2	VH3-23	1 del	CDR2
PPS 23F	3/7	018.6G5	κ/G2	VH3-7	2 ins	FRI
		027.064	κ/G2	VH3-23	1 ins	CDR2
		008.4B7	κ/G2	VH4-4	2 ins	CDR1

^aAntigen binding specificity of human antibody Fabs. ^bNumber of donors from which Fabs containing insertions or deletions were isolated over the number of donors analyzed. ^cGermline gene modified in the Fab. Kappa L chain nomenclature as described in [20], lambda L chain nomenclature as described in [21], H chain nomenclature as described in [23]. ^dLocation of the modification; CDR and frameworks regions are as described in [24].

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