Fig. 6From: LINC00909 up-regulates pluripotency factors and promotes cancer stemness and metastasis in pancreatic ductal adenocarcinoma by targeting SMAD4Overexpression of LINC00909 promotes apoptosis in PDAC cells. (A) Flow cytometry was conducted to detect apoptosis after overexpression of LINC00909 in PANC-1 cells. (B, C) Flow cytometry was utilized to detect apoptosis after knockdown of LINC00909 in PANC-1 and BxPC-3 cells. (D) Western blotting was performed to examine the expression of BCL-2 and PUMA after overexpression of LINC00909 in PANC-1 cells. (E, F) Western blotting was performed to examine the expression of BCL-2 and PUMA after knockdown of LINC00909 in PANC-1 and BxPC-3 cells. P-values were assessed using two-tailed t-tests in (A–C). (***P < 0.001; ****P < 0.0001). BCL-2: B-cell lymphoma-2; LINC00909: Long intergenic non-protein coding RNA 00909; PDAC: Pancreatic ductal adenocarcinomaBack to article page