| Family | Example of genes associated with CASS | Comments |
---|---|---|---|
1 | Reverse transriptase (RT) | VVA1544, PG1982, alr1468 | Fused to COG1518 in three occasions and a remnant of RT (Mbar_A1351 and MM3360) in M. barkeri and mazei genomes is located close to cas |
2 | PIN-domain | alr1560, ST0017, Ava_4168 | Ribonuclease |
3 | COG2442 | Ava_4167 | HTH domain, component of toxin-antitoxin system, probably targeting mRNA |
4 | COG1432 | MS0983 | Large family of proteins, predicted to be a phosphatase or a nuclease on the basis of sequence motifs which is shared by all three domain of life. In multidomain proteins in plants it is associated with C2H2 Zn-finger domain |
5 | PA2117-like | MS0982, MS0989 | An enzymatic domain, that is located in an operon with restriction-modification systems or in association with a diverged helicase |
6 | COG3645-like | ACIAD2479 | Homologs of phage anti-repressor Ant which is known to be inhibited by an antisense RNA |
7 | argonaute | MK1311 | Homolog of the eukaryotic argonaute protein, that are key player in RNA guided posttranscriptional regulation by siRNA and miRNA |
8 | COG1598/COG4226/HicB | MCA0653, MTH321 | Probably has an RNAseH-like fold, often fused to CopG-family of transcriptional regulators; forms a conserved operon with COG1724/hicA, which has the dsRBD-like fold; possible novel toxin-antitoxin module targeting mRNA |
9 | PUA-domain | LIC10933 | RNA binding domain |
10 | 3'-5' exonuclease | LcasA01001274 | Fused to COG1343 in Lactococcus bulgaricus and L.casei |
11 | COG1652 | TK0459 | Regulatory ATPase of AAA family fused to RecB-family nuclease; Predicted regulator of RNA metabolism |
12 | AbrB/MazE domain | TK0457, PAE0118 | DNA-binding domain, belongs to the same fold as MazE, which involved in toxin-antitoxin system |
13 | S1-domain | CaurDRAFT_2121 | Ribosomal protein S1-like RNA-binding domain, fused to RAMP domain |
14 | CSP-like | Rrub02003211 | Cold shock protein-like RNA-binding domain, fused to RAMP domain |